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Team:Illinois/Modeling
From 2009.igem.org
Modeling
sRNA regulation is a post-transcription regulation mechanism in which the mRNA transcript is degraded before it can be translated or preventing further translation. Traditionally regulation via sRNA is faster acting than transcription factors because there is no delay due to translation and protein folding.[1] For this reason, sRNAs are often found to regulate stress and environmental responses. For example, the sRNA, SgrS regulates PtsG, coding for two protein subunits EIIB and EIIC which are involved in the glucose phosphate stress response. [2]
The rate for protein translation after sRNA regulation is governed by the following simple differential equations. N is the number of particles, g is the growth rate, d is the rate of decay or half life of a particle, and gamma is the rate of dissociation of the mRNA-sRNA complex. Subtext s, m, x, and p, denotes sRNA, mRNA, the bound sRNA-mRNA molecule, and protein respectively.
The rate of change of the number of sRNA molecules is found by taking the growth rate, subtracting the number of sRNA molecules multiplied by the decay rate and subtracting the binding afinity to the mRNA multiplied by the number of mRNA molecules and the number of sRNA molecules. Questions about our Wiki page? Please email Dave Korenchan at korench1@illinois.edu.
