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Week Four
From 2009.igem.org
June 8th- 17th
What are we trying to do?
We want the bacteria to lyse, to kill itself from the inside.
Well then, to do that we had to get a lysis gene in it. The thing is, with the lysis gene needs to be turned on by this specific substance called a promoter
There are two kinds of promoters, ones that make the gene they're attached to , be 'on' all the time. Then, you have ones that turn on when you want them to, in our case, when you add a mystery enzyme.
Our Step-wise Process:
How we tried to do this:
Step 1 - Taking dry DNA from wells
Step 2 - Transforming competent cells
Step 3 - Picking a single colony.
Step 4 - Inoculating broth with Ampicillin-R (an antibiotic) and letting it grow for 18 hours.
Step 5 - Using the resulting culture for mini-prep. - a process used to purify plasmids and yields clean, usable DNA.
Step 6 - Digesting the DNA
Step 7 - Gel Electrophoresis
Step 8- Ligation
Step 9- Transformation and Inoculation
June 9th
Images of the various results attained:
 Agarose gel electrophoresis image of digested K112808 |
 Failure 1 |
 The pBAD Insert |
 The Failed Ligation Attempt |
