User:Annlesn

From 2009.igem.org

Ann's IGEM Notebook

7/22/09 and 7/23/09

  • Comp cell Protocol
  • Protocol for getting biobricks from the registry
  • Transformation Protocol
  • Transformation of RBS for Pu promoter and killer genes from the biobricks

7/24/09

  • Miniprep protocol
  • Miniprep of RBS for Pu promoter and killer genes

7/25/09

  • Nanodrop protocol
  • Digestion protocol
  • DNA concentrations for Pu promoter and killer genes
  • Digestion of RBS for Pu promoter and Bacteriophage lysis cassette from Brown 2008 with EcoRI and XbaI

7/27/09

  • Electrophoresis protocol
  • Gel of digested RBS for Pu promoter and Bacteriophage lysis cassette from Brown 2008
  • Sodium Benzoate Minimal Plate Recipe
  • Plates for Pu promoter colony PCR
  • Starting up Pu luciferase stab from the registry
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