"
13 July 2009
From 2009.igem.org
Aim:
- Ligation of tetR promoter and YFP, GFP, CFP.
Materials:
| Control 1 | Control 2 | YFP | GFP | CFP | |
|---|---|---|---|---|---|
| Ligase Buffer | 10 | 10 | 10 | 10 | 10 |
| Quick Ligase | 1 | 0 | 1 | 1 | 1 |
| dH2O | 6 | 7 | --- | --- | --- |
| Backbone | 3 | 3 | 3 | 3 | 3 |
| Promoter | --- | --- | 3 | 3 | 3 |
| FP | --- | --- | 3 | 3 | 3 |
| Total Volume | 20 | 20 | 20 | 20 | 20 |
Method:
- Add all the contents above into labelled eppendorfs, and spin down.
- Leave at room temperature for 30 min in order to fully ligate.
