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Minnesota-experimental/2 July 2009
From 2009.igem.org
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- Transformed TOP10 cells containing promoter constructs mutant at position 5 were screened using a fluorescent camera to select colonies producing GFP. This indicated that the cells within that colony contained plasmids with active promoters.
- Colonies which appeared green were transferred to a second antibiotic LB agar plate for further screening. The plate was marked into a numbered grid, and individual colonies were transferred into each square using a sterile micropipette tip. Cells were allowed to grow at 37 °C overnight.
