Team:TorontoMaRSDiscovery/18 August 2009

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August 18, 2009

  1. Tranfected ligation products into DH-5alpha cells and plated on old plates
  2. Miniprepped overnight culture of 7
  3. Digested Enc6, C (E,P)
  4. ran a gel with digests
  5. Digested Enc 6, C (X,S)
  6. Ran a gel with digests
  7. Gel extracted C (CIPed)
  8. Started overnight cultures for Tet, K plasmid
  9. Started overnight digest for Enc6
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