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Team:Wash U/Calendar-Home/14 July 2009
From 2009.igem.org
-Colony PCR of cells transformed with previous ligations
-PCR to add KpnI and BamHI sites to GFP
-Spectrophotometry of dbcOmega strains in ATCC media
-PCR to add BioBrick prefix and suffix to Cph8 and pRKCBC3
-Transformed S17 competent cells to check efficiency. OD = 0.115
-Performed Triligation and Silver Lab Ligations to fuse OmpC+GFP
-Started cultures of dbcOmega in LB+Strep in lieu of ATCC
Gel #1: Silver digestion protocol
1) Ladder
2) OmpC + pSB1A2 plasmid (2187 bp)
3) OmpC + pSB1A2 plasmid
4) GFP (876 bp)
5) GFP
6) Ladder
Gel #2: BioBrick (Triligation) digestion protocol
1) Ladder
2) OmpC (108 bp)
3) OmpC
4) Destination plasmid, pSB1K3 (2206 bp)
5) Dest. plasmid, pSB1K3
6) GFP (876 bp)
7) GFP
8) Ladder
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