Team:Wash U/Calendar-Home/14 September 2009

From 2009.igem.org


Monday September 14, 2009

-Made 1X M22 Media

-Made M22 agar plates 

   -Steaked out E. coli S17-1 pRKCBC3 on M22 agar to investigate growth on minimal media
   -Streaked out R. sphaeroides DBC omega on M22 agar to investigate growth on minimal media

-Made M22 strep 5ug/ml tet 5ug/ml agar plates for transconjugations 

   -Streaked out R. sphaeroides DBC omega on M22 strep 5ug/ml tet 5ug/ml agar plates as negative control

   -Streaked out E. coli S17-1 pRKCBC3 on M22 strep 5ug/ml tet 5ug/ml agar plates to investigate growth on antibiotic selective minimal media

-Performed Dr. Hunters transconjugation procedure using E. coli S17-1 pRKCBC3 and R. Sphaeroides DBC omega

-Performed Dr. Hunters transconjugation procedure using E. coli S17-1 pRKCBC3 with GFP and R. Sphaeroides DBC omega


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