Template:Team:KULeuven/15 September 2009/BlueLightReceptor

From 2009.igem.org

  1. miniprep of pBR322 (nanodrop: 24,5ng/µl)
  2. restriction digest of pBR322 with EcoRI and PstI
  3. gelelectrophoresis and extraction of pBR322 (nanodrop: 10,8ng/µl)
  4. ligation of pBR322 with ligA (blp + )
  5. electroporation of cells with LigY (attempt 3)
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