Template:Team:KULeuven/18 August 2009/VanillinReceptor

From 2009.igem.org

  • PCR purification miniprep and nanodrop of R on mutation 2
  • Start ethanol precipitation (overnight ethanol + sodium acetate) to create a better result (more purified product) of product R with mutation 202
  • PCR of the fragments of TOPO (W and Z) was done to check if TOPO worked. Gelelectrophoresis showed nothing so TOPO cloning failed again for virA.