Template:Team:KULeuven/31 August 2009/BlueLightReceptor
From 2009.igem.org
- A miniprep and RD (with EcoRI and PstI) were performed on the four colonies that were ented on Sunday.
- Nanoprop results
- RD results on gel: the right signals were detected
| Part | concentration (ng/μl) | 260/280 λ |
|
| LigA (1) | 110,6 | 1,92 |
|
| LigA (2) | 76,7 | 1,99 |
|
| LigA (3) | 84,8 | 2,07 |
|
| LigA (4) | 31,7 | 2,02 |
|
| | 104,3 | 2,10 |
|
- LigA was plated on LB medium. This will be the "motherplate" from which the glycerol stock and the testing cultures will be taken from.
- Electroporation of in competent cells. It was plated and put overnight in the incubator.
- In order to be able to grow vector , which contains the toxic ccdB gene, we need special cells which carry the gyrA462 mutation. This is strain DB3.1 from E.coli. These were plated and put in the incubator overnight.
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