Template:Team:KULeuven/3 September 2009/VanillinReceptor

From 2009.igem.org

  • The Y+K ligation was miniprepped and nanodropped to make later on a stock on glycerol
  • We decided to use for virA a puc19-vector in stead of TOPO, puc was digested with Xba1 and Ecor1 and virA was digested with EcoR1 and Spe1. This was done overnight.
  • electroporation + plating was done for X+K
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