"
August/12 August 2009
From 2009.igem.org
1.before Min prep
Checked the cell cultures transformed and plated out yesterday (8/10) for colony formation and made an approximate count of the number of colonies.
(plate number)-(location on plate) (no. of colonies)
[http://partsregistry.org/wiki/index.php?title=Part:BBa_C0077 1-14F] 50<
[http://partsregistry.org/wiki/index.php?title=Part:BBa_C0078 1-14H] 50<
[http://partsregistry.org/wiki/index.php?title=Part:BBa_I1466 1-23J] 100~
[http://partsregistry.org/wiki/index.php?title=Part:BBa_R0071 1-12C] 100~
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K118013 2-18F] 10
inoculate to iquid medium(5mL + Amp 5uL or Kan 25uL)
2.Transformation
to get new plasmid
each 4uL(DNA) , 1-2M , 1-12C : 2uL [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0034 1-2M] Amp [http://partsregistry.org/wiki/index.php?title=Part:BBa_C0161 2-8O] [http://partsregistry.org/wiki/index.php?title=Part:BBa_C2006 1-21F] [http://partsregistry.org/wiki/index.php?title=Part:BBa_F2621 2-21F] [http://partsregistry.org/wiki/index.php?title=Part:BBa_R0079 1-12A] [http://partsregistry.org/wiki/index.php?title=Part:BBa_C0171 2-8I] [http://partsregistry.org/wiki/index.php?title=Part:BBa_R0071 1-12C] [http://partsregistry.org/wiki/index.php?title=Part:BBa_F1610 2-24G] [http://partsregistry.org/wiki/index.php?title=Part:BBa_R0078 1-16G] [http://partsregistry.org/wiki/index.php?title=Part:BBa_I14017 1-18L] Kan [http://partsregistry.org/wiki/index.php?title=Part:BBa_I14015 2-11N] '8/11sample 10uL RBS+2-8M(LasR) EpsE+Terminater (after 65℃ , 10min)
3.LB plate
LB(500uL)+Amp(2.5mL) *25
+Kan(0.5mL) *25
4.Min prep
Result of Nanodrop concentration check
(sample No.) (concentration)
[http://partsregistry.org/wiki/index.php?title=Part:BBa_I719005 I179005] (1-15N) 19.0 ng/uL
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K091101 K091101] (2-6P) 27.0
[http://partsregistry.org/wiki/index.php?title=Part:BBa_J23100 J23100] (1-18C) 21.5
[http://partsregistry.org/wiki/index.php?title=Part:BBa_C0070 C0070] (1-12H) 48.0
[http://partsregistry.org/wiki/index.php?title=Part:BBa_B0015 B0015] (1-23L) 23.7
19.4
[http://partsregistry.org/wiki/index.php?title=Part:BBa_R0040 R0040] (1-6I) 9.3
[http://partsregistry.org/wiki/index.php?title=Part:BBa_I15009 I15009] (1-20F) 71.3
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K118013 K118013] (2-18F) 19.2
[http://partsregistry.org/wiki/index.php?title=Part:BBa_C0078 C0078] (1-14H) 49.9
[http://partsregistry.org/wiki/index.php?title=Part:BBa_I1466 I1466] (1-23J) 21.3
[http://partsregistry.org/wiki/index.php?title=Part:BBa_R0071 R0071] (1-12C) 10.7
[http://partsregistry.org/wiki/index.php?title=Part:BBa_C0077 C0077] (1-14F) 32
