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Duke University/18 June 2009
From 2009.igem.org
June 18, 2009
- E: Assembly PCR, Gels to check PCRs
2x Phusion mix 12.5 ul insert 7.14 ul H2O 5.36 ul 25 ul 98°C 30s 1x 98°C 10s 70-55°C 5s 72°C 30s 25x 72°C 2m 4°C infinity 1x
Fragment
| # | Name Size/Length (bp) | Concentration (ng/ul) | Mass (ng) | Volume (ul) | |
|---|---|---|---|---|---|
| 1 | pASKphaA | 4215 | 41 | 194 | 4.73 |
| 2 | phaB | 896 | 68 | 41 | 0.60 |
| 3 | Termcat2 | 1701 | 69 | 78 | 1.13 |
| 4 | phaC | 1872 | 126 | 86 | 0.68 |
| ~400 | 7.14 |
Length of plasmid: 8360 bp + 4 Histags * 6 aa * 3 bp = 8432 bp
- R: Smear because of misformed gel, no band
- C: Change conditions
