HKU-HKBU/30 July 2009
From 2009.igem.org
After spreading plates (A+ & C+) and growing overnight, both of the two plates grow.
17:10 Pre-culture bacteria with A+ & C+ plasmids in warm room (37℃) for overnight
Four bottles: 5mL LB Broth+5μL A++5μL C+