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Minnesota/9 July 2009
From 2009.igem.org
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Patrick
Today I received and analyzed the three models created yesterday, smad_ttn_gfp_u, smad_ttn_gfp_d, smad_ttn_gfp_d2. Below are the first two models, where the kinetic constant for reaction 10 (gfp -->) was raised and lowered by an order of magnitude (from 3.21E-05 1/s). The base is shown for comparison:
 Figure 1: Base TTN Model |
 Figure 2: Increased GFP Decomposition Rate (3.21E-04) |
 Figure 3: Decreased GFP Decomposition Rate (3.21E-06) |
As can be seen, there is a small difference when the GFP was increased, but no noticeable difference when GFP decomposition was decreased. The last model set GFP decomposition to zero in order to check whether a decrease would ever significantly affect the graph:
 Figure 4: GFP Decomposition Set to Zero |
As can be seen the elimination of GFP production doesn't have an effect. This makes sense since source literature says GFP decomposition is minor when compared to the dilution as a result of cell division.
