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Team:Kyoto/Cells in Cells/Notebook/week1
From 2009.igem.org
Contents
Monday
To Do on 24 July
- Miniprep (3-O-2, 3-A-4)
- Restriction Enzyme Digestion
- Electrophoresis
Results
Miniprep
- 3-O-2, 3-A-4 was minipreped.
Digestion
- Digested with EcoR1 / EcoR1 and Pst1 and run on a gel.
| NO. | Sample Name | Enzyme |
|---|---|---|
| 1 | 3-O-2 | Eco R1, PST1 |
| 2 | 3-A-4 | Eco R1, PST1 |
| 3 | 3-O-2 | Eco R1 |
| 4 | 3-A-4 | Eco R1 |
Electrophoresis
- Very clear bands were found. (See the gel image in right!)
- The bands are listed below.
| 3-O-2 | 3-A-4 | |
|---|---|---|
| Parts length / bp | 2837 | 487 |
| Plasmid length / bp | 3266 | 3266 |
Tuesday
To Do on 24 July
- Miniprep (3-O-2, 3-A-4)
- Restriction Enzyme Digestion
- Electrophoresis
Results
Miniprep
- 3-O-2, 3-A-4 was minipreped.
Digestion
- Digested with EcoR1 / EcoR1 and Pst1 and run on a gel.
| NO. | Sample Name | Enzyme |
|---|---|---|
| 1 | 3-O-2 | Eco R1, PST1 |
| 2 | 3-A-4 | Eco R1, PST1 |
| 3 | 3-O-2 | Eco R1 |
| 4 | 3-A-4 | Eco R1 |
Electrophoresis
- Very clear bands were found. (See the gel image in right!)
- The bands are listed below.
| 3-O-2 | 3-A-4 | |
|---|---|---|
| Parts length / bp | 2837 | 487 |
| Plasmid length / bp | 3266 | 3266 |
Wednesday
To Do on 24 July
- Miniprep (3-O-2, 3-A-4)
- Restriction Enzyme Digestion
- Electrophoresis
Results
Miniprep
- 3-O-2, 3-A-4 was minipreped.
Digestion
- Digested with EcoR1 / EcoR1 and Pst1 and run on a gel.
| NO. | Sample Name | Enzyme |
|---|---|---|
| 1 | 3-O-2 | Eco R1, PST1 |
| 2 | 3-A-4 | Eco R1, PST1 |
| 3 | 3-O-2 | Eco R1 |
| 4 | 3-A-4 | Eco R1 |
Electrophoresis
- Very clear bands were found. (See the gel image in right!)
- The bands are listed below.
| 3-O-2 | 3-A-4 | |
|---|---|---|
| Parts length / bp | 2837 | 487 |
| Plasmid length / bp | 3266 | 3266 |
Thursday
To Do on 24 July
- Miniprep (3-O-2, 3-A-4)
- Restriction Enzyme Digestion
- Electrophoresis
Results
Miniprep
- 3-O-2, 3-A-4 was minipreped.
Digestion
- Digested with EcoR1 / EcoR1 and Pst1 and run on a gel.
| NO. | Sample Name | Enzyme |
|---|---|---|
| 1 | 3-O-2 | Eco R1, PST1 |
| 2 | 3-A-4 | Eco R1, PST1 |
| 3 | 3-O-2 | Eco R1 |
| 4 | 3-A-4 | Eco R1 |
Electrophoresis
- Very clear bands were found. (See the gel image in right!)
- The bands are listed below.
| 3-O-2 | 3-A-4 | |
|---|---|---|
| Parts length / bp | 2837 | 487 |
| Plasmid length / bp | 3266 | 3266 |
Friday
To Do on 24 July
- Miniprep (3-O-2, 3-A-4)
- Restriction Enzyme Digestion
- Electrophoresis
Results
Miniprep
- 3-O-2, 3-A-4 was minipreped.
Digestion
- Digested with EcoR1 / EcoR1 and Pst1 and run on a gel.
| NO. | Sample Name | Enzyme |
|---|---|---|
| 1 | 3-O-2 | Eco R1, PST1 |
| 2 | 3-A-4 | Eco R1, PST1 |
| 3 | 3-O-2 | Eco R1 |
| 4 | 3-A-4 | Eco R1 |
Electrophoresis
- Very clear bands were found. (See the gel image in right!)
- The bands are listed below.
| 3-O-2 | 3-A-4 | |
|---|---|---|
| Parts length / bp | 2837 | 487 |
| Plasmid length / bp | 3266 | 3266 |
