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Team:Kyoto/CiC/Notebook/1005-1009
From 2009.igem.org
- 0907-0911
- constructing HIV-TAT-(LALAAAA)3-generator
- 0914-0918
- constructing HIV-TAT-(LALAAAA)3-generator
- 0921-0925
- constructing sig-GFP generator,sig-EGFP generator(HeLa)
- 0928-1002
- constructing sig-GFP generator,sig-EGFP generator(HeLa)
- 1005-1009
- constructing sig-GFP generator,sig-EGFP generator(HeLa)
- 1012-1016
- Observation
- 1019-1023
- Observation
1005-1009 : constructing sig-GFP generator,sig-EGFP generator(HeLa)
Monday, 05 October
To Do
No experiments were undertaken
Tuesday, 06 October
To Do
No experiments were undertaken
Wednesday, 07 October
To Do
- sig-GFP generator
- Restriction Enzyme
- ligation
- transformation
- sig-EGFP generator
- phosphorylation signal sequence primer
- anneling signal sequence primer
- ligation
- transformation
Results
Transformation
Too many colonies were observed that we could find no fignificant differences between the treated culuture the control. (091008)
Thursday, 08 October
To Do
- sig-GFP generator
- Restriction Enzyme
- ligation
- transformation
- sig-EGFP generator
- phosphorylation signal sequence primer
- anneling signal sequence primer
- ligation
- transformation
Results
Transformation
Sufficient,but not excessive number of colonies were observed,so we decided to move on to next step.(091010)
Friday, 09 October
To Do
- No experiments were undertaken
Saturday, 10 October
To Do
- sig-GFP
- miniprep
- Restriction Enzyme
- sig-EGFP(HeLa)
- miniprep
- Restriction Enzyme
Results
Plasmid construction is completed!!
Saturday, 11 October
To Do
- Yeast Mitochondria isolation
Results
- It is too difficult for us to isolate mitochondoria.
Sunday, 12 October
To Do
- No experiments were undertaken
