Template:Team:KULeuven/15 September 2009/VanillinReceptor

From 2009.igem.org

  • Because virA was now properly inserted in the pucvector we could now start mutagenesis on restriction sites 187 and 720. We did this with PCR. We used a protocol for site-directed-mutagenesis with the matching primers. PCR was done overnight
  • new ligation for virB was done