"
Template:Team:KULeuven/7 August 2009/VanillinReceptor
From 2009.igem.org
- Primers for mutagenesis have arrived
- We used the DNA parts from the second PCR to insert them into a TOPO-vector. The second PCR showed more results so that is why we used these fragments.
- LBmedium was made with X-gal to select cells afterwards.
- Plasmids were inserted in competent cells by heatshock and those cells were plated out on the X-gal medium.
