UCL London/Protocol/Restriction Enzyme Digestion

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Restriction Enzyme Digestion

Materials:

• Plasmid DNA: 10.5 µL/5 µL
• Restriction Enzymes (depend on the requirements of the parts)
  • EcoRI: 1 µL
  • PstI: 1 µL
  • SpeI: 1 µL
  • XbaI: 1 µL
• 10× NE buffer: 1.5 µL/1 µL (lid)
• BSA: 1 µL (lid)

Method:

1. Add all the contents listed above to labelled eppendorfs.
2. Spin for 30 sec.
3. Incubation at 37°C for 1hr.
4. Leave on ice before the gel electrophoresis.