Uppsala-Sweden/26 August 2009

From 2009.igem.org




Evaluating Competence

The results from yesterdays evaluation was disappointing. Only Hsin Ho's cells had any transformants at all. This seems a bit implausible so we performed a new evaluation, this time employing only our recently made Top10 cells. We inoculated using 1 and 5 µl of the pUC19 evaluation plasmid.

--Anders 20:33, 26 August 2009 (UTC)

Ethanol and Butanol Evolution

I transferred the Ethanol culture from #3 ( 2min UV exposure ) to 5% ethanol in 15ml BG11. A control with 0% was setup as well.

Furthermore I inocultated recovery culutures from 0,25%, 0,5% and 1 % in 1,5% Butanol BG11 medium(15ml total volume). A control with 0% Butanol was done as well

--Karl.brune 15:07, 26 August 2009 (UTC)

Retrieved from "http://2009.igem.org/Uppsala-Sweden/26_August_2009"