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Agarose Gels
From 2009.igem.org
(Difference between revisions)
(New page: {{UIUC09Temp}} {{Illinois}} {{IllExpNav}} ==Agarose Gel== *50mL 0.5x TBE buffer *Agarose (To determine number of grams to use, multiply volume (50mL) by percentage of gel. For example, u...) |
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*Agarose (To determine number of grams to use, multiply volume (50mL) by percentage of gel. For example, use 1.5g of agarose in a 3% agarose gel.) | *Agarose (To determine number of grams to use, multiply volume (50mL) by percentage of gel. For example, use 1.5g of agarose in a 3% agarose gel.) | ||
*2.5μL ethidium bromide | *2.5μL ethidium bromide | ||
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| + | When making a small gel use 50mL of TBE buffer. The dispenser is on the bench behind ours in a large Nalgene container. Use the graduated cylinder next to it, but only for the TBE buffer. | ||
Revision as of 20:25, 10 July 2009
Agarose Gel
- 50mL 0.5x TBE buffer
- Agarose (To determine number of grams to use, multiply volume (50mL) by percentage of gel. For example, use 1.5g of agarose in a 3% agarose gel.)
- 2.5μL ethidium bromide
When making a small gel use 50mL of TBE buffer. The dispenser is on the bench behind ours in a large Nalgene container. Use the graduated cylinder next to it, but only for the TBE buffer.
