Agarose Gels

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Agarose Gel

  • 50mL 0.5x TBE buffer
  • Agarose (To determine number of grams to use, multiply volume (50mL) by percentage of gel. For example, use 1.5g of agarose in a 3% agarose gel.)
  • 2.5μL ethidium bromide

When making a small gel use 50mL of TBE buffer. The dispenser is on the bench behind ours in a large Nalgene container. Use the graduated cylinder next to it, but only for the TBE buffer.

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