August/21 August 2009

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transformation

to get new plasmid

⑤(RBS+rhlR+terminator+promoter(reg))
⑦（RBS＋cinR)
⑧（RBS＋luxI)

check the result of transformation

2-8G     4
2-21F    0    
2-6O     2
2-13N   <15
⑤　　　　　　0

restriction and digestion

ⅰ　パーツ⑨ Insert H2O　　　　 　4 No.2　buffer 　2 ①ー３　 　12 EcoRI 　　1 SpeⅠ　　　　　　　　　　　　

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