"
August/23 August 2009
From 2009.igem.org
1.transformed check Checked the cell cultures transformed and plated out yesterday (8/22) for colony formation and made an approximate count of the number of colonies.
(ligation sample No.) (no. of colonies) inoculate to iquid medium(5mL + Amp 5uL or Kan 25uL)
2.digestion and ligation
'8/22 digestion sample
↓
gel electrophoresis
gel cut
↓
purification by [QIAquick Nucleotide Removal Kit]
↓
ligation
ligation DNA 44 10* buffer 5 ligation 1 total 50uL
↓
16°C 3~4hr
↓
transfromation
3.transfromation
ligation sample ⑤⑫⑬⑭⑮ 2-18H 1-8E 1-4E 2-16N 2-16L
