August/6 August 2009

From 2009.igem.org

(Difference between revisions)
(Design genetic circuits)
(Design genetic circuits)
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==Design genetic circuits==
==Design genetic circuits==
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We take notice of signal molecules (3OC6HSL,N-butylyl-HSL,3OH,C14:1-HSL,Al-1).So we
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We take notice of signal molecules. So I put them in order in the chart below.
 +
 
 +
 Indcer  signal moleculer  Receiver
 +
  LuxI ⇒     3OC6HSL      ⇒ LuxR
 +
  LasI ⇒  AI-1(3OC12HSL)  ⇒ LasR
 +
  CinI  ⇒   3OH,C14:1-HSl   ⇒ CinR

Revision as of 21:58, 6 August 2009

Contents

Morning Meeting

To do in lab

1. Transform & Selection

  • 培地作り
    • LB
    • LB Amp+
    • LB Kan+
  • Transformation
    • About 16 kinds of parts

2. Breeding

3. Refinement

To bring

  • Timer
  • Pen
  • Slipper

Design genetic circuits

We take notice of signal molecules. So I put them in order in the chart below.

 Indcer   signal moleculer   Receiver

  LuxI ⇒  3OC6HSL   ⇒ LuxR

  LasI ⇒  AI-1(3OC12HSL)  ⇒ LasR
 CinI  ⇒   3OH,C14:1-HSl   ⇒ CinR