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August/6 August 2009
From 2009.igem.org
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* CinI ⇒ 3OH,C14:1-HSl ⇒ CinR | * CinI ⇒ 3OH,C14:1-HSl ⇒ CinR | ||
* RhlI ⇒ AI-1(C4HSL) ⇒ RhlR | * RhlI ⇒ AI-1(C4HSL) ⇒ RhlR | ||
| - | * | + | * （例外）agrD synthesize AIP with agrB.AIp synthesize agrC . AgrA which is synthesized by agrC activate promoter. |
| - | + | ||
Revision as of 22:18, 6 August 2009
Contents |
Morning Meeting
To do in lab
1. Transform & Selection
- 培地作り
- LB
- LB Amp+
- LB Kan+
- Transformation
- About 16 kinds of parts
2. Breeding
3. Refinement
To bring
- Timer
- Pen
- Slipper
Design genetic circuits
We take notice of signal molecules. So I put them in order in the chart below.
Inducer signal moleculer Receiver
* LuxI ⇒ 3OC6HSL ⇒ LuxR * LasI ⇒ AI-1(3OC12HSL) ⇒ LasR * CinI ⇒ 3OH,C14:1-HSl ⇒ CinR * RhlI ⇒ AI-1(C4HSL) ⇒ RhlR * （例外）agrD synthesize AIP with agrB.AIp synthesize agrC . AgrA which is synthesized by agrC activate promoter.
