August/6 August 2009

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Contents

Morning Meeting

To do in lab

1. Transform & Selection

  • 培地作り
    • LB
    • LB Amp+
    • LB Kan+
  • Transformation
    • About 16 kinds of parts 
   We transform thesefollowing parts today.
      B0015 Plate 1 23L

2. Breeding

3. Refinement

To bring

  • Timer
  • Pen
  • Slipper

Design genetic circuits

We take notice of signal molecules. So I put them in order in the chart below.
　 *Inducer ⇒（synthesize) signal moleculer ⇒（synthesize) Receiver 

* LuxI ⇒　    3OC6HSL　     　⇒ LuxR
*  LasI ⇒　　AI-1(3OC12HSL) 　⇒　LasR
* CinI  ⇒  　3OH,C14:1-HSl  　⇒　CinR
* RhlI ⇒　　AI-1(C4HSL)  　　 ⇒　RhlR 
* （exception）agrD synthesize AIP with agrB.AIp synthesize agrC . AgrA which is synthesized by agrC activate promoter.

 From now on,We have to do what I mention below
 1.Search other cell-cell comunication system
 2.Check systems component completely
 3.Create new systems or reinforce cell funcution with additional ideas
 4.Search the effect of cross talk 

          written by Tadasi Nakamura
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