Duke University/18 June 2009

From 2009.igem.org

(Difference between revisions)
Line 16: Line 16:
72°C 2m
72°C 2m
4°C infinity 1x
4°C infinity 1x
 +
</pre>
-
Fragment # Name Size/Length (bp) Concentration (ng/ul) Mass (ng) Volume (ul)
+
Fragment
-
1 pASKphaA 4215 41 194 4.73
+
{| class="wikitable"
-
2 phaB 896 68 41 0.60
+
! #
-
3 Termcat2 1701 69 78 1.13
+
! Name Size/Length (bp)  
-
4 phaC 1872 126 86 0.68
+
! Concentration (ng/ul)  
 +
! Mass (ng)  
 +
! Volume (ul)
 +
|-
 +
| 1 || pASKphaA || 4215 || 41 || 194 || 4.73
 +
|-
 +
| 2 || phaB || 896 || 68 || 41 || 0.60
 +
|-
 +
| 3 || Termcat2 || 1701 || 69 || 78 || 1.13
 +
|-
 +
| 4 || phaC || 1872 || 126 || 86 || 0.68
~400 7.14
~400 7.14
-
</pre>
+
|}
-
 
+
Length of plasmid: 8360 bp + 4 Histags * 6 aa * 3 bp = 8432 bp
Length of plasmid: 8360 bp + 4 Histags * 6 aa * 3 bp = 8432 bp
*R: Smear because of misformed gel, no band
*R: Smear because of misformed gel, no band
*C: Change conditions
*C: Change conditions

Revision as of 16:44, 21 October 2009

Return

June 18, 2009

  • E: Assembly PCR, Gels to check PCRs 
2x Phusion mix 12.5 ul 
insert 7.14 ul 
H2O 5.36 ul 
25 ul 

98°C 30s 1x
98°C 10s
70-55°C 5s 
72°C 30s 25x
72°C 2m
4°C infinity 1x

Fragment

# Name Size/Length (bp) Concentration (ng/ul) Mass (ng) Volume (ul)
1 pASKphaA 4215 41 194 4.73
2 phaB 896 68 41 0.60
3 Termcat2 1701 69 78 1.13
4 phaC 1872 126 86 0.68

~400 7.14

Length of plasmid: 8360 bp + 4 Histags * 6 aa * 3 bp = 8432 bp

  • R: Smear because of misformed gel, no band
  • C: Change conditions
Retrieved from "http://2009.igem.org/Duke_University/18_June_2009"