EPF-Lausanne/7 July 2009
From 2009.igem.org
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(New page: ===Wet Lab=== We have to grow the 3 strains generously sent by [mailto:j.beatty@ubc.ca Tom Beatty] The three strains are : :*''R.Palustris'' CEA001 (wild type) ; should be grown on LB med...) |
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Then, a miniprep was done with both cultures. | Then, a miniprep was done with both cultures. | ||
A LOVTAP plasmid aliquot was done, a TrpR plasmid aliquot was done, located in the -20 fridge, 2nd floor. | A LOVTAP plasmid aliquot was done, a TrpR plasmid aliquot was done, located in the -20 fridge, 2nd floor. | ||
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+ | ===Cloning Strategy=== | ||
+ | To design plasmids : software Vector NTI |
Revision as of 15:37, 27 July 2009
Wet Lab
We have to grow the 3 strains generously sent by Tom Beatty
The three strains are :
- R.Palustris CEA001 (wild type) ; should be grown on LB medium only
- R.Palustris BPHP1+ ; should be grown on LB with gentamycin (100 micrograms/ml)
- E.Coli DH10B (pBPH/hmu0) ; should be grown on LB with gentamycin (20 micorgrams/ml)
The transformed LOVTAP and TrpR worked well (N.B. the plasmid of TrpR is pUC19 so the antibiotic resistance is Amp -> see below)
We did the glycerol stock, located in the -80 fridge, first floor of the iGEM compartement.
Then, a miniprep was done with both cultures. A LOVTAP plasmid aliquot was done, a TrpR plasmid aliquot was done, located in the -20 fridge, 2nd floor.
Cloning Strategy
To design plasmids : software Vector NTI