File:Chiba Labwork 09Sep09.jpg

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0800 main culture
 
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    divided injection 12.5ml of the culture to 10 50ml centrifuging tubes
 
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    WASH 3 times
 
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    Add 12.5ml new culture
 
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    Centrifuge the 1st tube 3000rpm 5min 20℃ and pick the upernatant
 
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    Do same operation every 15min to 2nd~10th tubes
 
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    after all, mix the supernatant and solid media at 1:1
 
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    make 10 concentration varieties of solid media
 

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