HKU-HKBU/21 July 2009

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14:00 Sub-culture to warm room (37℃) for 100min, until OD~0.4-0.5
Follow the protocol of electro-transformation. Transform 1μL plasmid (C+) to bacteria.
15:40 Take bacteria out from warm room (OD is too small). Go on subculture in warm room.