Hansi Liu's notebook

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7.22 posted ‎‎Aug 19, 2009 1:35 AM‎‎ by Hansi Liu Transient Cells 1. Empty vector+pMAX 

   CH12=0_CH34=100nMfMLP_CH56=100nMCNO

2. pBR64(hM4)+pMAX 

   CH1=0_CH2=50nMCNO_CH3=100nMCNO_CH4=500nMCNO_CH5=1uMCNO_CH6=1.5uMCNO
   CH1=0_CH2=50nMCNO_CH3=100nMCNO_CH4=500nMCNO_CH5=1uMCNO_CH6=1.5uMCNO
   CH1=0_CH2=50nMCNO_CH3=100nMCNO_CH4=1uMCNO_CH5=1.5uMCNO_CH6=100fMLP

7.31 posted ‎‎Aug 19, 2009 1:22 AM‎‎ by Hansi Liu 

FACS sorted hM4 stable cell line

hM4 High 

1. starve for 1.5hr_CH1=16nMCNO_CH2=48nMCNO_CH3=100nMCNO_CH4=1uMCNO_CH5=1.5uMCNO_CH6=2uMCNO
2. starve for 1.5hr_CH1=16nMCNO_CH2=48nMCNO_CH3=100nMCNO_CH4=1uMCNO_CH5=1.5uMCNO_CH6=2uMCNO
3. starve for 1.5hr_CH12=0_CH34=1uMCNO_CH56=100nMfMLP

hM4 Low 

1. CH1=16nMCNO_CH2=48nMCNO_CH3=100nMCNO_CH4=1uMCNO_CH5=1.5uMCNO_CH6=2uMCNO
2. CH1=16nMCNO_CH2=48nMCNO_CH3=100nMCNO_CH4=1uMCNO_CH5=1.5uMCNO_CH6=2uMCNO
3. CH1=16nMCNO_CH2=48nMCNO_CH3=100nMCNO_CH4=1uMCNO_CH5=1.5uMCNO_CH6=2uMCNO
4. CH23=0_CH456=100nMfMLP

7.27 posted ‎‎Aug 19, 2009 1:18 AM‎‎ by Hansi Liu 

FACS sorted hM4 stable cell lines
hm4High
1. CH12=0_CH34=100nMCNO_CH56=100nMfMLP
2. CH1=0_CH2=16nMCNO_CH3=16nMCNO_CH4=1uMCNO_CH5=5uMCNO_CH6=5uMCNO
3. CH1=0_CH2=16nMCNO_CH3=48nMCNO_CH4=100nMCNO_CH5=1uMCNO_CH6=2uMCNO
4. CH1=0_CH2=16nMCNO_CH3=48nMCNO_CH4=100nMCNO_CH5=1uMCNO_CH6=1.5uMCNO
5. CH1=0_CH2=16nMCNO_CH3=48nMCNO_CH4=100nMCNO_CH5=1uMCNO_CH6=1.5uMCNO
6. CH1=0_CH2=48nMCNO_CH3=100nMCNO_CH4=1uMCNO_CH5=1.5uMCNO_CH6=2uMCNO
7. CH1=0_CH23=16nMCNO_CH4=1uMCNO_CH56=5uMCNO

7.21 posted ‎‎Aug 19, 2009 1:16 AM‎‎ by Hansi Liu 

WT HL-60 
1. CH12=0to0_CH34_0to4nMfMLP_CH56=0to8nMfMLP
2. CH12=0to32nMfMLP_CH34=0to64nMfMLP_CH56=0to80nMfMLP
3. CH1=110nMfMLP_CH2=120nMfMLP_CH3=130nMfMLP_CH4=140nMfMLP_CH5=150nMfMLP_CH6=0

7.20 posted ‎‎Aug 19, 2009 1:14 AM‎‎ by Hansi Liu [ updated ‎‎Aug 19, 2009 1:29 AM‎‎ ] 

WT HL-60
CH123=0to0_CH456=0to100nMCNO
Transient Cells
pBR64(hM4)+pMAX
CH1=0_CH2=0to16nMCNO_CH3=0to100nMCNO_CH4=0to160nMCNO_CH5=0to1uMCNO_CH6=0to100nMfMLP

7.17 posted ‎‎Aug 19, 2009 1:08 AM‎‎ by Hansi Liu 

EZ-Taxiscanning:

WT HL-60 

1. CH23=0to0_CH456=0to100nMCNO
2. CH123=0to0_CH456=0to100nMfMLP

hM4 Stable Cell Line
1. High expression level
   CH1=0to0_CH2=0to16nMCNO_CH3=0to48nMCNO_CH4=0to100nMCNO_CH5=0to160nMCNO_CH6=0to1uMCNO
2. Medium expression level
   CH1=0to0_CH2=0to16nMCNO_CH3=0to48nMCNO_CH4=0to100nMCNO_CH5=0to160nMCNO_CH6=0to1uMCNO
3. Low expression level
   CH1=0to0_CH2=0to16nMCNO_CH3=0to48nMCNO_CH4=0to100nMCNO_CH5=0to160nMCNO_CH6=0to1uMCNO

7.14 posted ‎‎Aug 19, 2009 1:03 AM‎‎ by Hansi Liu 

EZ-Taxiscanning:

WT HL-60
1. CH123=0to0nMfMLP_CH456=0to100nMfMLP_starve1.5hr
2. CH123=0to0nMfMLP_CH456=0to100nMfMLP_6um chip_starve1hr
3. CH12=0to16nMfMLP_CH34=0to48nMfMLP_CH56=0to160nMfMLP_starve1.5hr

hM4 Stable Cell Line
CH1=0to0nMCNO_CH2=100pMCNO_CH3=1nMCNO_CH4=10nMCNO_CH5=50nMCNO_CH6=100nMCNO_5uMchip

7.28 posted ‎‎Jul 2, 2009 10:45 PM‎‎ by Hansi Liu [ updated ‎‎Aug 19, 2009 1:35 AM‎‎ ] 

Transient Cells

1. Empty vector+pMAX
   CH12=0_CH34=1uMCNO_CH56=100fMLP

2. pBR64(hM4)+pMAX
   CH1=0_CH2=50nMCNO_CH3=100nMCNO_CH4=1uMCNO_CH5=2uMCNO_6=5uMCNO
   CH1=0_CH2=50nMCNO_CH3=100nMCNO_CH4=1uMCNO_CH5=2uMCNO_6=5uMCNO

7.2 posted ‎‎Jul 2, 2009 10:45 PM‎‎ by Hansi Liu [ updated ‎‎Jul 3, 2009 12:44 AM‎‎ ] 

11:00
Ready to get an ID card !

13:30-14:30
Angi taught us how to analysis scan result(cell tracking) using a cool Matlab program written by herself.

15:00-
Completed the safety training courses on-line. Ready to do experiments~

later:
Mini Prep: 8 samples from Eric.
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