Illinois/10 July 2009
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(New page: {{UIUC09Temp}} {{Illinois}} {{IllinoisCalendar}} == July 10 == '''3'-Acting sRNAs''' Today we performed a midiprep to extract the plasmid DNA from dH5α. [https://2009.igem.org/Team:I...) |
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+ | '''sRNA Library''' | ||
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+ | Hopefully now the ligation will be working. After many many attempts we got a decent looking gel! The resolution isnt very good. This was an attempt to fit both the insert and the vector on the same gel but that didn't work at all. Its 1% with a 1kbp ladder. | ||
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+ | [[Image:8laneligation_roomtemp.png]] | ||
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+ | The large, slow bands are hopefully the ligated plasmids. The ones just below that don't look like they worked. They look reasonably bright. Each lane is a variant of Insert:Vector ratio, ranging from 3:1 to 15:1 which were all ligated at room temperature for 4 hrs. (Protocol called for 1hr) | ||
'''3'-Acting sRNAs''' | '''3'-Acting sRNAs''' |
Revision as of 16:31, 14 July 2009
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July 10
sRNA Library
Hopefully now the ligation will be working. After many many attempts we got a decent looking gel! The resolution isnt very good. This was an attempt to fit both the insert and the vector on the same gel but that didn't work at all. Its 1% with a 1kbp ladder.
The large, slow bands are hopefully the ligated plasmids. The ones just below that don't look like they worked. They look reasonably bright. Each lane is a variant of Insert:Vector ratio, ranging from 3:1 to 15:1 which were all ligated at room temperature for 4 hrs. (Protocol called for 1hr)
3'-Acting sRNAs
Today we performed a midiprep to extract the plasmid DNA from dH5α.