Illinois/10 July 2009

From 2009.igem.org

(Difference between revisions)
(July 10)
 
(One intermediate revision not shown)
Line 1: Line 1:
{{UIUC09Temp}}
{{UIUC09Temp}}
{{Illinois}}
{{Illinois}}
 +
<html>
 +
<div id="uicontentbox">
 +
</html>
{{IllinoisCalendar}}
{{IllinoisCalendar}}
Line 28: Line 31:
[https://2009.igem.org/Team:Illinois/Notebook Back to Notebook page]
[https://2009.igem.org/Team:Illinois/Notebook Back to Notebook page]
 +
<html>
 +
</div>
 +
</html>
 +
{{IllinoisBottomNav}}

Latest revision as of 21:21, 20 July 2009

Click to go to the Illinois home page



June
MTWTFSS
1 2 3 4 5 6 7
8 9 10 11 12 13 14
15 16 17 18 19 20 21
22 23 24 25 26 27 28
29 30
July
MTWTFSS
    1 2 3 4 5
6 7 8 9 10 11 12
13 14 15 16 17 18 19
20 21 22 23 24 25 26
27 28 29 30 31
August
MTWTFSS
          1 2
3 4 5 6 7 8 9
10 11 12 13 14 15 16
17 18 19 20 21 22 23
24 25 26 27 28 29 30
31

July 10

sRNA Library

Hopefully now the ligation will be working. After many many attempts we got a decent looking gel! The resolution isnt very good. This was an attempt to fit both the insert and the vector on the same gel but that didn't work at all. Its 1% with a 1kbp ladder.

8laneligation roomtemp.png

The large, slow bands are hopefully the ligated plasmids. The ones just below that don't look like they worked. They look reasonably bright. Each lane is a variant of Insert:Vector ratio, ranging from 3:1 to 15:1 which were all ligated at room temperature for 4 hrs. (Protocol called for 1hr)

3'-Acting sRNAs

Today we performed a midiprep to extract the plasmid DNA from dH5α.

Hybrid Promoter

Today we came up with a schematic of a possible decoder:
IllinoisDecoderjuly10.png


We looked more closely at the biobricks: BBa_I14032, BBa_R0040, BBa_K137125, BBa_K091101.

BBa_R0040 and BBa_K091101 were available to us in the registry distribution so we transformed these into cells to test them.


Back to Notebook page

Questions about our Wiki page? Please email us at illinoisiGEM@gmail.com.