Illinois/21 July 2009

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July 21

Hybrid Promoter

Finished overnight digestion and digested with SpeI

Modeling

We may have determined why our plasmid backbone pSB3K3 has been turning up in gels as 2kbp rather than 2.75kbp. There is a Parts Registry link that seems to indicate that the pSB3K3 backbone with the two Biobricks we have been using has problems. It also suggested an alternative Biobrick in the kit. We ran PCRs on the old Biobrick with this backbone, the Biobrick suggested by the Parts Registry, and a third Biobrick with the backbone from the 2008 Parts Distribution. Unfortunately, our gel indicated that the PCRs were unsuccessful. We will have to try again tomorrow.

PSB3K3 PCR 7-21.jpg


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