Illinois/22 July 2009
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(New page: {{UIUC09Temp}} {{Illinois}} <html> <div id="uicontentbox"> </html> {{IllinoisCalendar}} == July 22 == '''Modeling''' We retried PCRs for the pSB3K3 plasmid backbone, using two samples o...) |
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+ | '''Hybrid Promoter''' | ||
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+ | The gel that was run to inactivate the digestion looked awful. | ||
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+ | [[Image:UI09_DR_promoter_digestion_7-22.jpg|200px|thumb|center]] | ||
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+ | Reexamined protocol from the fermentas website and decided to attempt a double digest using Spe1 first and then EcoRI | ||
'''Modeling''' | '''Modeling''' |
Latest revision as of 01:37, 29 July 2009
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July 22
Hybrid Promoter
The gel that was run to inactivate the digestion looked awful.
Reexamined protocol from the fermentas website and decided to attempt a double digest using Spe1 first and then EcoRI
Modeling
We retried PCRs for the pSB3K3 plasmid backbone, using two samples of template: Spring 2009 Plate 2 Well 15L and Spring 2008 Plate 1014 Well 1F. We ran four different PCRs, two for each under two different PCR programs: the program for amplifying PJU-334 and the program suggested for amplifying pSB3K3. Our gel indicated that none of the four PCRs worked.