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Illinois/25 June 2009
From 2009.igem.org
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(New page: {{UIUC09Temp}} {{Illinois}} {{IllinoisCalendar}} == June 25 == '''Team Name 1''' Journal entry text goes here. '''Team Name 2''' Journal entry text goes here. [https://2009.igem.org/...) |
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== June 25 == | == June 25 == | ||
| - | ''' | + | '''Modeling''' |
| - | + | We created a working program in SimBiology that roughly models our decoder. In the program, each gene is regulated by two sRNAs. The sRNA synthesis rates are assumed to be constant for now, whereas in reality these rates will depend on input concentrations and promoter activity. We verified by changing sRNA synthesis rates that combinations of two sRNAs resulted in production of the correct fluorescent protein. | |
| - | + | [[Image:UI092to4diagram.jpg|450px]][[Image:UI092to4graphs.jpg|450px]] | |
| - | + | We cultured cells overnight in anticipation that we will be able to perform a fluorescence reading tomorrow. | |
[https://2009.igem.org/Team:Illinois/Notebook Back to Notebook page] | [https://2009.igem.org/Team:Illinois/Notebook Back to Notebook page] | ||
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Latest revision as of 21:18, 20 July 2009
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June 25
Modeling
We created a working program in SimBiology that roughly models our decoder. In the program, each gene is regulated by two sRNAs. The sRNA synthesis rates are assumed to be constant for now, whereas in reality these rates will depend on input concentrations and promoter activity. We verified by changing sRNA synthesis rates that combinations of two sRNAs resulted in production of the correct fluorescent protein.
We cultured cells overnight in anticipation that we will be able to perform a fluorescence reading tomorrow.
