Illinois/30 June 2009

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June 30

sRNA Library

We started creating primers for 5 new sRNA's and each of their respective target sequences:


sRNA's / target sequences

  dicF  /  ftsZ
  dsrA  /  hns
  rhyB  /  sodB
  spot42/  galK
  rseX  /  ompC and ompA
  gcvB  /  dppa and oppa

We PCR'ed all of the genes listed above except for ompC and ompA, and we obtained bands for each of the target sequences. However, for the sRNA's, we only obtained bands for gcvB and dicF.


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