Illinois/30 June 2009
From 2009.igem.org
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June 30
sRNA Library
We started creating primers for 5 new sRNA's and each of their respective target sequences:
sRNA's / target sequences
dicF / ftsZ dsrA / hns rhyB / sodB spot42/ galK rseX / ompC and ompA gcvB / dppa and oppa
We PCR'ed all of the genes listed above except for ompC and ompA, and we obtained bands for each of the target sequences. However, for the sRNA's, we only obtained bands for gcvB and dicF.
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