Illinois/6 July 2009

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== July 6 ==
== July 6 ==
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'''sRNA Library'''
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We performed another PCR of all of the new sRNA's, and we ran a gel with the 5 sRNA's from last week. 
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[[Image:redo1.jpg]]
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 +
Lane 1    100bp ladder
 +
    2    ftsZ
 +
    3    ftsZ*
 +
    4    oppA
 +
    5    oppA*
 +
    6    dppa
 +
    7    dppa*
 +
    8    hns
 +
    9    galK
 +
    10  sodB
 +
 +
[[Image:redo2.jpg]]
 +
 +
Lane 1    100bp ladder
 +
    2    dicF
 +
    3    dicF*
 +
    4    gcvB
 +
    5    gcvB*
 +
    6    rseX
 +
    7    spot42
 +
    8    ryhB
 +
    9    dsrA
 +
 +
The genes that have an asterisk are the samples from July 2st, and none of them seemed to show bands.  SodB, spot42, and dsrA also didn't work.
 +
 +
We ran a digestion with the PCR products that did work, however we did not do the PCR purification.
'''Modeling'''
'''Modeling'''
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[https://2009.igem.org/Team:Illinois/Notebook Back to Notebook page]
[https://2009.igem.org/Team:Illinois/Notebook Back to Notebook page]
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{{IllinoisBottomNav}}

Latest revision as of 21:21, 20 July 2009

Click to go to the Illinois home page



Home
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Biobricks Notebook

June
MTWTFSS
[http://2009.igem.org/Illinois/1_June_2009 1] [http://2009.igem.org/wiki/index.php?title=Illinois/2_June_2009&action=edit 2] [http://2009.igem.org/wiki/index.php?title=Illinois/3_June_2009&action=edit 3] [http://2009.igem.org/wiki/index.php?title=Illinois/4_June_2009&action=edit 4] [http://2009.igem.org/wiki/index.php?title=Illinois/5_June_2009&action=edit 5] [http://2009.igem.org/wiki/index.php?title=Illinois/6_June_2009&action=edit 6] [http://2009.igem.org/wiki/index.php?title=Illinois/7_June_2009&action=edit 7]
[http://2009.igem.org/wiki/index.php?title=Illinois/8_June_2009&action=edit 8] [http://2009.igem.org/wiki/index.php?title=Illinois/9_June_2009&action=edit 9] [http://2009.igem.org/wiki/index.php?title=Illinois/10_June_2009&action=edit 10] [http://2009.igem.org/wiki/index.php?title=Illinois/11_June_2009&action=edit 11] [http://2009.igem.org/wiki/index.php?title=Illinois/12_June_2009&action=edit 12] [http://2009.igem.org/wiki/index.php?title=Illinois/13_June_2009&action=edit 13] [http://2009.igem.org/wiki/index.php?title=Illinois/14_June_2009&action=edit 14]
[http://2009.igem.org/Illinois/15_June_2009 15] [http://2009.igem.org/Illinois/16_June_2009 16] [http://2009.igem.org/Illinois/17_June_2009 17] [http://2009.igem.org/Illinois/18_June_2009 18] [http://2009.igem.org/Illinois/19_June_2009 19] [http://2009.igem.org/wiki/index.php?title=Illinois/20_June_2009&action=edit 20] [http://2009.igem.org/wiki/index.php?title=Illinois/21_June_2009&action=edit 21]
[http://2009.igem.org/wiki/index.php?title=Illinois/22_June_2009&action=edit 22] [http://2009.igem.org/Illinois/23_June_2009 23] [http://2009.igem.org/Illinois/24_June_2009 24] [http://2009.igem.org/Illinois/25_June_2009 25] [http://2009.igem.org/Illinois/26_June_2009 26] [http://2009.igem.org/wiki/index.php?title=Illinois/27_June_2009&action=edit 27] [http://2009.igem.org/wiki/index.php?title=Illinois/28_June_2009&action=edit 28]
[http://2009.igem.org/Illinois/29_June_2009 29] [http://2009.igem.org/Illinois/30_June_2009 30]
July
MTWTFSS
    [http://2009.igem.org/Illinois/1_July_2009 1] [http://2009.igem.org/Illinois/2_July_2009 2] [http://2009.igem.org/Illinois/3_July_2009 3] [http://2009.igem.org/wiki/index.php?title=Illinois/4_July_2009&action=edit 4] [http://2009.igem.org/wiki/index.php?title=Illinois/5_July_2009&action=edit 5]
[http://2009.igem.org/Illinois/6_July_2009 6] [http://2009.igem.org/Illinois/7_July_2009 7] [http://2009.igem.org/wiki/index.php?title=Illinois/8_July_2009&action=edit 8] [http://2009.igem.org/Illinois/9_July_2009 9] [http://2009.igem.org/Illinois/10_July_2009 10] [http://2009.igem.org/wiki/index.php?title=Illinois/11_July_2009&action=edit 11] [http://2009.igem.org/wiki/index.php?title=Illinois/12_July_2009&action=edit 12]
[http://2009.igem.org/Illinois/13_July_2009 13] [http://2009.igem.org/Illinois/14_July_2009 14] [http://2009.igem.org/Illinois/15_July_2009 15] [http://2009.igem.org/Illinois/16_July_2009 16] [http://2009.igem.org/Illinois/17_July_2009 17] [http://2009.igem.org/wiki/index.php?title=Illinois/18_July_2009&action=edit 18] [http://2009.igem.org/wiki/index.php?title=Illinois/19_July_2009&action=edit 19]
[http://2009.igem.org/Illinois/20_July_2009 20] [http://2009.igem.org/Illinois/21_July_2009 21] [http://2009.igem.org/Illinois/22_July_2009 22] [http://2009.igem.org/Illinois/23_July_2009 23] [http://2009.igem.org/Illinois/24_July_2009 24] [http://2009.igem.org/wiki/index.php?title=Illinois/25_July_2009&action=edit 25] [http://2009.igem.org/wiki/index.php?title=Illinois/26_July_2009&action=edit 26]
[http://2009.igem.org/wiki/index.php?title=Illinois/27_July_2009&action=edit 27] [http://2009.igem.org/wiki/index.php?title=Illinois/28_July_2009&action=edit 28] [http://2009.igem.org/wiki/index.php?title=Illinois/29_July_2009&action=edit 29] [http://2009.igem.org/wiki/index.php?title=Illinois/30_July_2009&action=edit 30] [http://2009.igem.org/wiki/index.php?title=Illinois/31_July_2009&action=edit 31]
August
MTWTFSS
          [http://2009.igem.org/wiki/index.php?title=Illinois/1_August_2009&action=edit 1] [http://2009.igem.org/wiki/index.php?title=Illinois/2_August_2009&action=edit 2]
[http://2009.igem.org/wiki/index.php?title=Illinois/3_August_2009&action=edit 3] [http://2009.igem.org/wiki/index.php?title=Illinois/4_August_2009&action=edit 4] [http://2009.igem.org/wiki/index.php?title=Illinois/5_August_2009&action=edit 5] [http://2009.igem.org/wiki/index.php?title=Illinois/6_August_2009&action=edit 6] [http://2009.igem.org/wiki/index.php?title=Illinois/7_August_2009&action=edit 7] [http://2009.igem.org/wiki/index.php?title=Illinois/8_August_2009&action=edit 8] [http://2009.igem.org/wiki/index.php?title=Illinois/9_August_2009&action=edit 9]
[http://2009.igem.org/wiki/index.php?title=Illinois/10_August_2009&action=edit 10] [http://2009.igem.org/wiki/index.php?title=Illinois/11_August_2009&action=edit 11] [http://2009.igem.org/wiki/index.php?title=Illinois/12_August_2009&action=edit 12] [http://2009.igem.org/wiki/index.php?title=Illinois/13_August_2009&action=edit 13] [http://2009.igem.org/wiki/index.php?title=Illinois/14_August_2009&action=edit 14] [http://2009.igem.org/wiki/index.php?title=Illinois/15_August_2009&action=edit 15] [http://2009.igem.org/wiki/index.php?title=Illinois/16_August_2009&action=edit 16]
[http://2009.igem.org/wiki/index.php?title=Illinois/17_August_2009&action=edit 17] [http://2009.igem.org/wiki/index.php?title=Illinois/18_August_2009&action=edit 18] [http://2009.igem.org/wiki/index.php?title=Illinois/19_August_2009&action=edit 19] [http://2009.igem.org/wiki/index.php?title=Illinois/20_August_2009&action=edit 20] [http://2009.igem.org/wiki/index.php?title=Illinois/21_August_2009&action=edit 21] [http://2009.igem.org/wiki/index.php?title=Illinois/22_August_2009&action=edit 22] [http://2009.igem.org/wiki/index.php?title=Illinois/23_August_2009&action=edit 23]
[http://2009.igem.org/wiki/index.php?title=Illinois/24_August_2009&action=edit 24] [http://2009.igem.org/wiki/index.php?title=Illinois/25_August_2009&action=edit 25] [http://2009.igem.org/wiki/index.php?title=Illinois/26_August_2009&action=edit 26] [http://2009.igem.org/wiki/index.php?title=Illinois/27_August_2009&action=edit 27] [http://2009.igem.org/wiki/index.php?title=Illinois/28_August_2009&action=edit 28] [http://2009.igem.org/wiki/index.php?title=Illinois/29_August_2009&action=edit 29] [http://2009.igem.org/wiki/index.php?title=Illinois/30_August_2009&action=edit 30]
[http://2009.igem.org/wiki/index.php?title=Illinois/31_August_2009&action=edit 31]

July 6

sRNA Library

We performed another PCR of all of the new sRNA's, and we ran a gel with the 5 sRNA's from last week.


Redo1.jpg

Lane 1 100bp ladder 

    2    ftsZ
    3    ftsZ*
    4    oppA
    5    oppA*
    6    dppa
    7    dppa*
    8    hns
    9    galK
    10   sodB

Redo2.jpg

Lane 1 100bp ladder 

    2    dicF
    3    dicF*
    4    gcvB
    5    gcvB*
    6    rseX
    7    spot42
    8    ryhB
    9    dsrA

The genes that have an asterisk are the samples from July 2st, and none of them seemed to show bands. SodB, spot42, and dsrA also didn't work.

We ran a digestion with the PCR products that did work, however we did not do the PCR purification.

Modeling

Transformations of GFP and the reference standard promoter were successful. We successfully miniprepped the DNA for both Biobricks.


Back to Notebook page

Questions about our Wiki page? Please email us at illinoisiGEM@gmail.com.

Retrieved from "http://2009.igem.org/Illinois/6_July_2009"