Illinois/6 July 2009
From 2009.igem.org
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July 6
sRNA Library
We performed another PCR of all of the new sRNA's, and we ran a gel with the 5 sRNA's from last week.
Lane 1 100bp ladder
2 ftsZ 3 ftsZ* 4 oppA 5 oppA* 6 dppa 7 dppa* 8 hns 9 galK 10 sodB
Lane 1 100bp ladder
2 dicF 3 dicF* 4 gcvB 5 gcvB* 6 rseX 7 spot42 8 ryhB 9 dsrA
The genes that have an asterisk are the samples from July 2st, and none of them seemed to show bands. SodB, spot42, and dsrA also didn't work.
We ran a digestion with the PCR products that did work, however we did not do the PCR purification.
Modeling
Transformations of GFP and the reference standard promoter were successful. We successfully miniprepped the DNA for both Biobricks.
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