Indiana/6 July 2009

From 2009.igem.org

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Grow liquid culture to prepare for mini prep
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'''Grow liquid culture to prepare for mini prep'''
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'''Preparation of Media'''
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Preparation of Media:
The modified plasmid is kan resistant and the parts pulled from the tool kit are amp resistant, so both media were made.
The modified plasmid is kan resistant and the parts pulled from the tool kit are amp resistant, so both media were made.

Revision as of 19:06, 19 July 2009

Grow liquid culture to prepare for mini prep

Preparation of Media:

The modified plasmid is kan resistant and the parts pulled from the tool kit are amp resistant, so both media were made.

Kan media:

50 mL LB

50 uL KAN

Amp media:

50 mL LB

50 uL amp


add 3 mL of media to a tube

transfer 1 colony into each tube

shake @ 37 C o/n