Indiana/6 July 2009

From 2009.igem.org

Revision as of 18:59, 19 July 2009 by Twahlig (Talk | contribs)
(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)

Grow liquid culture to prepare for mini prep

Preparation of Media:

The modified plasmid is kan resistant and the parts pulled from the tool kit are amp resistant, so both media were made.

Kan media: 50 mL LB 50 uL KAN

Amp media: 50 mL LB 50 uL amp

add 3 mL of media to a tube transfer 1 colony into each tube shake @ 37 C o/n