Indiana/6 July 2009

From 2009.igem.org

Revision as of 19:07, 19 July 2009 by Twahlig (Talk | contribs)

Grow liquid culture to prepare for mini prep

Preparation of Media:

The modified plasmid is kan resistant and the parts pulled from the tool kit are amp resistant, so both media were made.

Kan media:

50 mL LB

50 uL KAN


Amp media:

50 mL LB

50 uL amp


add 3 mL of media to a tube

transfer 1 colony into each tube

shake @ 37 C o/n