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LOVTAP
From 2009.igem.org
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<br> Sequence from NCBI : [[Media:Sequence_du_Trp_repressor.txt]] | <br> Sequence from NCBI : [[Media:Sequence_du_Trp_repressor.txt]] | ||
<br> Design a biobrick that coexpresses LOVTAP and mCherry (after Trp operon) when LOVTAP bind the Trp operon. Design a switch on/off read out. | <br> Design a biobrick that coexpresses LOVTAP and mCherry (after Trp operon) when LOVTAP bind the Trp operon. Design a switch on/off read out. | ||
| - | <br> | + | |
| - | ;- Do a mutational assay to change specificity of LOVTAP: | + | <br>;- Do a mutational assay to change specificity of LOVTAP: |
:Direct mutational assay: Insert mutation in specific sites thanks to the known structure of LOVTAP (already modeled on computer) | :Direct mutational assay: Insert mutation in specific sites thanks to the known structure of LOVTAP (already modeled on computer) | ||
:Indirect mutational assay: Random mutations, then selection of the "right" protein according to a set of selected conditions | :Indirect mutational assay: Random mutations, then selection of the "right" protein according to a set of selected conditions | ||
Revision as of 14:44, 2 June 2009
To find
- Sequence of the 12 mutant fusion proteins or LOV domain AND TrpR (separately) => Pubmed
To do
- - Find the exact genetic circuit for Trp repressor Nath
- - Write an e-mail to Strickland et al to ask Basile first shot; I think we could then look at it all together
Sequence (of all 12 fusion proteins)
Purification protocol
Digest assay protocol
Idea of letter :Media:letter_strickland.pdf
- - Biobricks
Look for a (or many) paper(s) that characterizes E.Coli Trp repressor, and find the Trp operon sequence Mél
Summary of what characterizes E.Coli Trp repressor : Media:The_tryptophan_biosynthetic_pathway.pdf
One good article : RNA-based_regulation_of_genes_of_tryptophan_synthesis_an_degradation.pdf
Sequence from NCBI : Media:Sequence_du_Trp_repressor.txt
Design a biobrick that coexpresses LOVTAP and mCherry (after Trp operon) when LOVTAP bind the Trp operon. Design a switch on/off read out.
;- Do a mutational assay to change specificity of LOVTAP:
- Direct mutational assay: Insert mutation in specific sites thanks to the known structure of LOVTAP (already modeled on computer)
- Indirect mutational assay: Random mutations, then selection of the "right" protein according to a set of selected conditions
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