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METU-Gene
From 2009.igem.org
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Revision as of 22:16, 22 September 2009
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Wound Healing velocity will increase as described in the video. Effects of Thymosin beta 4 are similar with synthesis of Epidermal Growth Factor and Keratinocyte Growth Factor our system synthesizing.
Although we plan to use Thymosin beta 4 next year, Our Wound Healing Mechanism will be upgraded with this future implication.
Thymosin beta 4 which consists of 68 a.a accelerated skin wound healing in a rat model of a full thickness wound where the epithelial layer was destroyed. When Tb4 was applied topically to the wound or injected into the animal, epithelial layer restoration in the wound was increased 42% by day four and 61% by day seven, after treatment, compared to untreated. Furthermore, Tb4 stimulated collagen deposition in the wound and angiogenesis. Tb4 accelerated keratinocyte migration, resulting in the wound contracting by more than 11%, compared to untreated wounds, to close the skin gap in the wound. An analysis of skin sections (histological observations) showed that the Tb4 treated wounds healed faster than the untreated. Proof of accelerated cell migration was also seen in vitro, where Tb4 increased keratinocyte migration two to threefold, within four to five hours after treatment, compared to untreated keratinocytes.
