Minnesota-experimental/2 July 2009

From 2009.igem.org

Revision as of 03:12, 22 October 2009 by Linkri (Talk | contribs)
(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)
Back to Notebook Home
Go to Previous Day (July 1)Go to Next Day (July 3)

- Transformed TOP10 cells containing promoter constructs mutant at position 5 were screened using a fluorescent camera to select colonies producing GFP. This indicated that the cells within that colony contained plasmids with active promoters.

- Colonies which appeared green were transferred to a second antibiotic LB agar plate for further screening. The plate was marked into a numbered grid, and individual colonies were transferred into each square using a sterile micropipette tip. Cells were allowed to grow at 37 °C overnight.