http://2009.igem.org/wiki/index.php?title=Minnesota/10_July_2009&feed=atom&action=historyMinnesota/10 July 2009 - Revision history2024-03-28T12:31:54ZRevision history for this page on the wikiMediaWiki 1.16.5http://2009.igem.org/wiki/index.php?title=Minnesota/10_July_2009&diff=34602&oldid=prevPatsmad at 19:19, 30 July 20092009-07-30T19:19:47Z<p></p>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><br></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">'''Patrick'''<br></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">Yesterday's results showed that lowing GFP production, while it had the potential to shift the amount of time it takes the system to reach steady state, it couldn't shift it enough even when the decomposition was set to zero. This was because cell division resulted in dilution that would make GFP reach a steady state after about 3 hours.<br></ins></div></td></tr>
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<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">The key to shifting this curve more is perhaps to change how the tetR2 molecules exist as complexes. In the TTN system tetR2 can exist by itself, as tetR2:tetO1, tetR2:aTc, tetR2:nsDNA, tetR2:tetO1:aTc, etc. about 11 different complexes. Today I focused on determining in which complexes tetR2 mostly existed as at the end of a simulation<br></ins></div></td></tr>
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<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">The results, obtained using a m-file I created, showed that at low aTc concentrations tetR2 was mostly bound to non-specific DNA (nsDNA), and floating about unbound (tetR2). At high concentrations of aTc all of it existed as tetR2:aTc2, which makes sense<br></ins></div></td></tr>
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<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">In trying to fit the experimental data, these results will be taking into consideration<br></ins></div></td></tr>
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<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">'''Ben'''</ins><br></div></td></tr>
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</table>Patsmadhttp://2009.igem.org/wiki/index.php?title=Minnesota/10_July_2009&diff=32977&oldid=prevBswiniar at 19:35, 29 July 20092009-07-29T19:35:55Z<p></p>
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</table>Bswiniarhttp://2009.igem.org/wiki/index.php?title=Minnesota/10_July_2009&diff=30262&oldid=prevBswiniar at 15:48, 28 July 20092009-07-28T15:48:57Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|'''[[Team:Minnesota/Modeling| Back to Notebook Home]]'''</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|'''[[Team:Minnesota/Modeling| Back to Notebook Home]]'''</div></td></tr>
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</table>Bswiniarhttp://2009.igem.org/wiki/index.php?title=Minnesota/10_July_2009&diff=30251&oldid=prevBswiniar: New page: {|style="align:left" width="965" |- |''' Back to Notebook Home''' |- |'''Go to Previous Day (July 9)'''|| width=158|'''[[Minnesota/11...2009-07-28T15:47:27Z<p>New page: {|style="align:left" width="965" |- |'''<a href="/Team:Minnesota/Modeling" title="Team:Minnesota/Modeling"> Back to Notebook Home</a>''' |- |'''<a href="/Minnesota/9_July_2009" title="Minnesota/9 July 2009">Go to Previous Day (July 9)</a>'''|| width=158|'''[[Minnesota/11...</p>
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