Minnesota/29 June 2009

From 2009.igem.org

(Difference between revisions)
Line 8: Line 8:
I switched over today to looking at making the "leakiness" equations for the TTN model. Initially I would represent the leakiness with two equations:<br>
I switched over today to looking at making the "leakiness" equations for the TTN model. Initially I would represent the leakiness with two equations:<br>
 +
{| class="wikitable" style="text-align:center" border = "1"
 +
|-
 +
! Rxn #
 +
! Reaction
 +
! Forward Kinetic Constant
 +
|-
 +
|46||RNAp + lacP + tetO1:tetR2 + tetO2 -> RNAp:lacP||1E+07
 +
|-
 +
|47||RNAp + lacP + tetO1 + tetO2:tetR2 -> RNAp:lacP||1E+07
 +
|}<br>
 +
The aTc complexes don't have to be represented since the leakiness is for aTc=0 in particular. Also a third leakiness equation could be made, one where both tetO1:tetR2 and tetO2:tetR2 exist, but I chose to add this later if necessary. I expect the results tomorrow.<br>
'''Ben'''<br>
'''Ben'''<br>
[[Image:Tnnatcvariesleaky9.jpg|480px]][[Image:Tnnexperiment.jpg|480px]]<br>
[[Image:Tnnatcvariesleaky9.jpg|480px]][[Image:Tnnexperiment.jpg|480px]]<br>

Revision as of 22:17, 30 July 2009

Back to Notebook Home
Go to Previous Day (June 26)Go to Next Day (June 30)

Patrick
I switched over today to looking at making the "leakiness" equations for the TTN model. Initially I would represent the leakiness with two equations:

Rxn # Reaction Forward Kinetic Constant
46RNAp + lacP + tetO1:tetR2 + tetO2 -> RNAp:lacP1E+07
47RNAp + lacP + tetO1 + tetO2:tetR2 -> RNAp:lacP1E+07

The aTc complexes don't have to be represented since the leakiness is for aTc=0 in particular. Also a third leakiness equation could be made, one where both tetO1:tetR2 and tetO2:tetR2 exist, but I chose to add this later if necessary. I expect the results tomorrow.

Ben
Tnnatcvariesleaky9.jpgTnnexperiment.jpg